常用单位及转换

一、Metric   Prefixes
G = giga = 109
M = mega = 108
k = kilo = 103
m = milli = 10-3
μ= micro = 10-6
n = nano = 10-9
p = pico = 10-12
f = femto = 10-15
a = atto = 10-18
二、重量换算
1μg=10-6 g
1ng =10-9 g
1pg =10-12g
1fg =10-15 g

三、A260单位的换算
1A260单位的双链DNA=50μg/ml
1A260单位的单链RNA=40μg/ml
1A260单位的单链DNA=40μg/ml
1A260单位的寡糖苷酸=33μg/ml

四、重量与摩尔的换算
1μg的 1kb DNA = 1.52pmol
1μg的pBR322 DNA =0.36pmol
1pmol of 1,000bp DNA =0.66μg
1pmol of pUC18/19 DNA(2.686bp) =1.77μg
1pmol of pBR322 DNA(4,361 bp) =2.88μg
1pmol of M13mp18/19 DNA(7,249bp) =4.78μg
1pmol of DNA（48，502 bp）=32.01μg

五、核酸碱基对与分子量的换算
1kb的双链DNA（钠盐）=6.6×105D
1kb的单链DNA（钠盐）=3.3×105D
1kb单链RNA（钠盐）=3.4×105D
脱氧核苷酸的平均分子量=324.5D

六、常见核酸的分子量

七、核酸碱基分子量

AMP         347.2
CMP         323.2
GMP         363.3
UMP         324.2
damp      331.2
dCMP      307.2
dGMP      347.2
dTMP         322.2

八、蛋白质重量的换算

100pmoles of 100,000 dalton protein=10μg
100pmoles of 500,000 dalton protein=5μg
100pmoles of 10,000 dalton protein=1μg

九、蛋白质与核酸的换算

lkb DNA=333个氨基酸的编码能力
333个氨基酸3.7×104D（道尔顿）
10,000D蛋白质=270bp   DNA
30,000D蛋白质=810bp   DNA
50,000D蛋白质=1.35kb   DNA
100,000D蛋白质=2.7kb   DNA